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A) The schematic diagram of crosstalk between treated macrophages and ESCs. B,C) The percentage of α‐SMA positive cells evaluated by immunofluorescence staining in each group. D–F) The mRNA expression of α‐SMA, Col <t>III,</t> <t>and</t> <t>FN1</t> was detected by RT‐PCR in different groups. G,H) The concentrations of Col III and FN1 in cell supernatant were measured by ELISA. Data are presented as mean ± SD. * p < 0.05 compared to the control group, ** p < 0.05 compared to the LPS+GS group, *** p < 0.05 compared to the LPS+siRNA@EVs/GS group, n = 6.
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Figure 2. Using <t>ELISA</t> to detect the effect of tuberostemonine on the expressions of FN, Col I, Col <t>III,</t> and HYP in cell culture supernatant(A)(B). (##p < .01 vs Ctrl group; **p < .01 vs TGF-β1 group, n = 6).
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A) The schematic diagram of crosstalk between treated macrophages and ESCs. B,C) The percentage of α‐SMA positive cells evaluated by immunofluorescence staining in each group. D–F) The mRNA expression of α‐SMA, Col III, and FN1 was detected by RT‐PCR in different groups. G,H) The concentrations of Col III and FN1 in cell supernatant were measured by ELISA. Data are presented as mean ± SD. * p < 0.05 compared to the control group, ** p < 0.05 compared to the LPS+GS group, *** p < 0.05 compared to the LPS+siRNA@EVs/GS group, n = 6.

Journal: Advanced Science

Article Title: Gene Therapy for Inflammatory Cascade in Intrauterine Injury with Engineered Extracellular Vesicles Hybrid Snail Mucus‐enhanced Adhesive Hydrogels

doi: 10.1002/advs.202410769

Figure Lengend Snippet: A) The schematic diagram of crosstalk between treated macrophages and ESCs. B,C) The percentage of α‐SMA positive cells evaluated by immunofluorescence staining in each group. D–F) The mRNA expression of α‐SMA, Col III, and FN1 was detected by RT‐PCR in different groups. G,H) The concentrations of Col III and FN1 in cell supernatant were measured by ELISA. Data are presented as mean ± SD. * p < 0.05 compared to the control group, ** p < 0.05 compared to the LPS+GS group, *** p < 0.05 compared to the LPS+siRNA@EVs/GS group, n = 6.

Article Snippet: The enzyme‐linked immunosorbent assay (ELISA) kits for tumor necrosis factor‐α (TNF‐α) and interleukin‐1 beta (IL‐1β) were purchased from NeoBioscience Technology Co. Ltd (Shenzhen, China), and for collagen (Col) III and fibronectin (FN1) were bought from AMEKO (Lianshuo Technology Co. Ltd, Zhejiang, China) and CUSABIO (CSB‐E04553r, https://www.cusabio.com/ , Hubei, China).

Techniques: Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Figure 2. Using ELISA to detect the effect of tuberostemonine on the expressions of FN, Col I, Col III, and HYP in cell culture supernatant(A)(B). (##p < .01 vs Ctrl group; **p < .01 vs TGF-β1 group, n = 6).

Journal: International journal of immunopathology and pharmacology

Article Title: Tuberostemonine may alleviates proliferation of lung fibroblasts caused by pulmonary fibrosis.

doi: 10.1177/03946320241274225

Figure Lengend Snippet: Figure 2. Using ELISA to detect the effect of tuberostemonine on the expressions of FN, Col I, Col III, and HYP in cell culture supernatant(A)(B). (##p < .01 vs Ctrl group; **p < .01 vs TGF-β1 group, n = 6).

Article Snippet: Mouse tumor necrosis factor α (TNF-α) ELISA kit (#CSB-E04741m), mouse macrophage colonystimulating factor (M-CSF) ELISA kit (#CSB-E04659 m), human fibronectin ELISA kit (CSB-E04551h), human collagen type III ELISA kit (#CSB-E04799h), human collagen type I ELISA kit (#CSB-E08082 h) were purchased from CUSABIO (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture

Figure 7. (A) (B) Employing ELISA to detect the secretion levels of IL-1β, IL-2, IL-6, TNF-a, IFN-y, M-CSF, MIP-2 and MCP-1 in animal plasma (##p < .01 vs Ctrl group; **p < .01 vs Model group, n = 10).

Journal: International journal of immunopathology and pharmacology

Article Title: Tuberostemonine may alleviates proliferation of lung fibroblasts caused by pulmonary fibrosis.

doi: 10.1177/03946320241274225

Figure Lengend Snippet: Figure 7. (A) (B) Employing ELISA to detect the secretion levels of IL-1β, IL-2, IL-6, TNF-a, IFN-y, M-CSF, MIP-2 and MCP-1 in animal plasma (##p < .01 vs Ctrl group; **p < .01 vs Model group, n = 10).

Article Snippet: Mouse tumor necrosis factor α (TNF-α) ELISA kit (#CSB-E04741m), mouse macrophage colonystimulating factor (M-CSF) ELISA kit (#CSB-E04659 m), human fibronectin ELISA kit (CSB-E04551h), human collagen type III ELISA kit (#CSB-E04799h), human collagen type I ELISA kit (#CSB-E08082 h) were purchased from CUSABIO (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics